Ranil+Fernando

I can pickup the plates later today or tomorrow. I am not sure if they allow you to put any of the actual experiment items on the display. Please check with Ms. Anita.

Hi Mr. Fernando, We finished counting our samples. We are ready to give them back to you to be disposed of. We were also wondering if we would be able to keep a few plates to put on the table at the actual science fair? Please let us know what you think. -Anna, Krystine, Megan, and David 5/6/12

Mr. Fernando, When can we get the samples back to you? -Emily and Lauren 5/6/12

Mr. Fernando, Change of plans. We are unable to meet tomorrow morning but we can meet tomorrow after school, as long as we can be finished sometime around 4:00. Is that possible? Emily and Lauren 5/2/12

Mr. Fernando, Sorry for the late response! Can you bring the results tomorrow (Thursday, May 3) morning? Emily and Lauren 5/2/12

Mr. Fernando, Can you please bring the samples tomorrow morning? Thank you. Anna, Krystine, Megan, and David 5/2/12

Hi Emily, Lauren, Anna, Krystine, Megan and David,

The results of the experiment are ready to be tabulated. I can bring these to you if you wish. They have to be handled carefully so I need to explain to you what to do. Let me know if you have time during after school. One of the parents from each group will need to be there as well. Ranil 4/30/2012

Yes that can be done. Ranil 4/27/2012

Should you take pictures of the bacteria in the incubator? - Emily and Lauren 4/27/12

Hi Mr. Fernando, We're going to do the experiment on the morning of Wednesday 4/25 and bring them to the office on that day for you to pick up. Thanks! -Emily Woodward and Lauren Mitchell 4/23/12

Hi Mr. Fernando,

If we were planning on taking samples this Thursday(4/26/12), would you be able to pick them up that morning? If we do this when would you suggest we count the colonies? Please let us know if you have any suggestions. Thank you!

-Anna, Megan, Krystine, and David 4/23/12

**//Hi Emily and Lauren,//**

**//My apologies for not getting back to you sooner. Yes that is fine. I should be able to get the swabs tomorrow and leave it for you at the school. Probobly at the front office to your attention. Please do not sample during the weekend but instead wait until next week. If you do it one of the mornings and bring it to school. Please have them call me (they have my number). I can arrainge to have it picked up and tested by the lab the same day. It will then take about 7 days to get the results.//**

**//Ranil Fernando, 4/19/2012//**

**//Hi Anna, Krystine, Megan and David,//**

**//I will also have your water containers delivered to the school tomorrow.//** Please do not sample during the weekend but instead wait until next week. If you do it one of the mornings and bring it to school. Please have them call me (they have my number). I can arrainge to have it picked up and tested by the lab the same day. It will then take about 7 days to get the results.

Ranil Fernando 4/19/2012 **//Here is the Science Fair Paperwork collected for your groups this week.... Brian and Jodi Burda//**

Mr. Fernando, We are going to narrow it down to only door handles, light switches, and cabinet/drawer handles in 8 different rooms (4 bedrooms and 4 bathrooms), and 1 swab for our control, which is a total of 25 swabs. Is that too much? We would like to get our supplies as soon as possible. Thanks! -Emily W. and Lauren M. 4/11/12

Mr. Fernando, So, we'll be starting our project soon and are ready for our supplies. We're planning on sampling door handles, light switches, cabinet/drawer handles, trash cans, and corners of floors and walls. We want to test these areas in the bedrooms and bathrooms in our houses. For our control group, we are going to have one swab that we don't alter. Your thoughts? -Emily W. and Lauren M. 2/3/12

Hi Mr. Fernando, We are planning on having 6 water sources. Would it be alright if we tested each 3 times or would 2 be sufficient? We would only need sterile containers for 3 of the sources because the others are bottled waters. Your thoughts?

Thanks, Anna, Krystine, Megan, and David

Mr. Fernando, So far, we are planning to swab floors, walls, hard surfaces (desk, counter, shelf), door handles and light switches. Is there anything else you think that we should test? Also, where can we get the supplies we need? As for agar, probably nutrient agar or LB agar. Your thoughts?

-Emily and Lauren

Hi Anna, Krystine, Megan and David,

You are on the correct track. You need to use a laboratory that is certified to test water. The test you need is the "Hetertrophic Plate Count". This can be done by pour plate procedure or membrane filteration procedure. It is best to get the water sampled and to the lab as soon as possible (few hours) and no longer than 30 hours. Remember that the lab needs time to test it once you drop the samples off. The laboratory you use should be able to provide the water collections samples as well. Once you know how many types of water (ocean, drinking, bottled, swimming pool) and how many replicates you plan to test they should be able to give you a price.

Thanks

Ranil

Hi Mr. Fernando, Do you know where we would be able to get "sterile" containers? Also, we have looked up one website to see how to test water. This is it: http://www.ehow.com/how_5492432_test-water-bacteria.html Would this be sufficient? Thank you, Anna L. and the other group members 3/4/12

Hi Anna, Krystine, Megan and David,

You would need to use a "sterile" container with a lid to collect the samples of water. After that you will need to test the water to prove your hypothesis. Have you done research to see how you would conduct your experiment?

Ranil

Hi Emily and Lauren,

Sounds like you are off to a good start. You will need to decide which agar will be used in the petri dish. You should include that in your research. You should consider what surfaces you will sample such as floors, walls, door handles, etc.

Ranil

Hello. My name is Anna Leoncio. I contacted you before about our group. We're all really excited to work with you. We wanted to know what kind of container we would be able to put our samples in. Our topic is "What type of water will contain the most bacteria?" Also, we were curious about how you would suggest for us to measure our results. Thank You very much. -Anna Leoncio, Krystine B., Megan M., and David M. 3/1/12

Lauren and I recently met together and completed our bibliography and research questions. We will complete our research paper in school sometime this week.

Hi! My name is Emily Woodward, and I, along with Lauren Mitchell will be working with you for our science project. Our topic question is: Is there more bacteria in a bathroom or a bedroom? For our experiment, we were planning on using a petri dish to measure amounts of bacteria in these areas and compare the results. As for research, we are most likely going to be using a book and/or the internet. So far, we haven't began much work on our project, but Lauren and I are planning to meet together soon.

Mrs. Kreide, Lauren and I could not remember exactly which title we chose for our project and submitted. What was it? -Lauren M. and Emily W.

The title was "Are there more bacteria in a bedroom or a bathroom?" You are not stuck with that title, please feel free to change it as you see fit. Cannot wait to see the results, Mrs. Kreide